Human Peripheral CD8+ CD103+ T-lymphocyte Transmigration Through Inverted Cryptosporidium Parvum Sporozoite Infected HCT-8 Cell Monolayers

Publisher Summary Studies of spontaneous and/or experimental cryptosporidiosis indicated that T-cell mediated immunity as the chief host defense against Cryptosporidium parvum. Although limited information is available on interactions between mucosal C. parvum and T-lymphocyte compartments, data suggest involvement of both systemic and gut-associated lymphoid tract lymphocytes. Although origin of intestinal intraepithelial lymphocytes (ilEL) has not been yet well defined, it is generally believed that recruitment of ilEL to the intestinal epithelium needs extravasation of lymphocytes from the blood stream, followed by migration into the epithelia compartment. Intestinal lEL express aE(CD103)p7 (>90%), while this integrin is expressed by less than 2% of peripheral blood lymphocytes. In this context, the present study that is elucidated in this chapter was addressed to obtain an in vitro model for the study of interactions between T-lymphocytes and C. parvum- infected entreocytic cells. The results showed that parasites of all stages, including high ratios of meronts and gametes were observed, which suggest that complete parasite development was achieved. The experimental system described in this chapter supported the sexual and asexual C. parvum developments within three days in HCT-8 inverted monolayers and provided cell-cell contact between enterocytic cells and peripheral blood lymphocytes. Present experiments in this chapter suggest that inverted enterocytic cell culture models are suitable for further study of interactions between lymphocytes and C.parvum- infected enterocytic cells.