Identification and biocontrol effects of antagonistic bacterium DS-1 strain against Fusarium oxysporum f. sp. cucumerinum.

2010 
The taxonomic status, growth promoting and biocontrol effects against Fusarium oxysporum f. sp. cucumerinum of antagonistic bacterium DS-1 strain, screened by co-culture on PDA plates from rhizospheric soil of continuously cropped cucumber in solar greenhouse, morphology, physiological and biochemical characteristics, and 16S rDNA sequencing of the strain were determined, the effects on cucumber seed germination and seedling fusarium wilt biocontrol were studied. The results showed that: The 16S rDNA sequence of DS-1 strain shared 100% homology with Brevibacillus brevis from GenBank. Based on the results of 16S rDNA sequencing, morphological and cultural observation as well as physiological and biochemical determination, DS-1 strain was identified as Brevibacillus brevis, with GenBank accession number EF108303. DS-1 strain promoted germination of cucumber seeds, with significant biocontrol effects against fusarium wilt of cucumber seedlings by 84.92%.
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