Determination precise du rapport lecithine/ sphingomyeline dans le liquide amniotique: Proposition d'une methode de reference

Abstract Pulmonary maturity of the fetus can be evaluated by the lecithin/sphingo-myelin (L/S) ratio in amniotic fluid. To existing methods of lipid extraction, precipitation with acetone and chromatography, we add a simple and accurate estimation of sphingomyelins (S) and precipitated lecithins (Lp) without acid digestion. The method is reproducible (C.V.