Stable oxygen and carbon isotope profiles in an invasive bivalve (Corbicula fluminea) in North Carolina watersheds

Abstract The modern invasive bivalve Corbicula fluminea was collected in 2006 from three sites with different land uses located in a North Carolina River Basin. The primary objective was to describe the δ 18 O and δ 13 C profiles of C. fluminea shells under various land use conditions. An additional aim was to evaluate whether growth patterns of C. fluminea form seasonally. Annual shell growth patterns were measured from the umbo to the margin and co-varied with estimates of ambient water temperature, corresponding to seasonal variation. The C. fluminea growth patterns as translucent bands (slower growth) appeared to form during winter months and opaque bands (rapid growth) formed during summer. A mixed model analysis (ANOVA) showed a significant site level effect of δ 18 O and δ 13 C profiles examined among sites ( F  = 17.1; p  = 0.003). A second model showed a borderline significant site effect among profiles with variability more pronounced at the urban site, Crabtree Creek ( p  = 0.085). Previous habitat assessment ratings and water chemistry measurements suggested that the urban site was more impacted by storm water runoff. Understanding δ 18 O and δ 13 C SHELL profiles and shell growth patterns of the invasive bivalve ( C. fluminea ) may help establish a framework for using these animals as biomonitors to record water temperature and nutrient pollution.