Abstract 12263: The Role of Glucagon-Like Peptide-1 in the Immediate Improvement of Endothelial-Protective HDL Functions After Roux-en-Y Gastric Bypass in Obese Patients

Introduction: Roux-en-Y gastric bypass (RYGB) reduces body weight (BW) and cardiovascular (CV) mortality in morbidly obese patients (pts). We showed in rats that glucagon-like peptide-1 (GLP-1) contributes to improve endothelial vasorelaxation early after RYGB in a BW loss-independent manner. Hypothesis: Here, we investigated in rats and patients whether obesity-induced HDL dysfunction improves after RYGB by a GLP-1-dependent mechanism. Methods: Diet induced obese male Wistar rats undergoing RYGB received vehicle (RYv); sham-operated ad libitum fed rats received vehicle (ALv) or the GLP-1 analog liraglutide (AL-lira; 0.2 mg/kg BID). HDL was isolated after 8 days. In parallel, HDL was also isolated from 28 healthy subjects and 28 morbidly obese pts (BMI >40) before, 14 days and 12 weeks after RYGB. In endothelial cells stimulated with HDL, we assessed nitric oxide (NO) production by DAF-2 fluorescence and endothelial NO synthase (eNOS) protein dimerization. Moreover, we measured paraoxonase-1 (PON-1) antio...