Sero-prevalence and risk factor analysis of Theileria equi infection in equids from different agro-climatic zones of Punjab (India) by Indirect Immunofluorescence Antibody test

Abstract In the Indian sub-continent, equine piroplasmosis (EP) is an imperative tick-borne disease of equids predominately caused by obligatory intra-erythrocytic protozoa Theileria equi . Present exploration is the first epidemiological survey report based on Indirect Immunofluorescence Antibody test (IFAT). A total of 120 equines (98 horses and 22 donkeys/mules) from five districts of two main agro-climatic zones of Punjab were screened. The overall prevalence was 58.33%, with western plane zone at higher infection risk for T. equi (74.50%, OR = 7.45, 95% CI = 62.24–85.76); followed by western zone (46.38%, OR = 4.63, 95% CI = 34–57.7). District wise highest and lowest prevalence was recorded in district Fazilka (80.76%) and Bathinda (35.29%) respectively. IFAT revealed elevated risk of infection of T. equi in the farms with inappropriate management structure. The risk factor connected with the type of host species had an odds ratio of 1.67 for donkeys/mules versus horses for T. equi infection. Followed by presence/absence of tick on animal. This study reveals the endemicity of EP in Punjab with prominence of various odds of infection, associated with latent infection of T. equi detectable by serological techniques as it was noticed that western plane zone and western zone are more prone to EP infection.