Identification of a leaf-rust resistance gene in species of Aegilops L., synthetic forms, and introgression lines of common wheat

DNA samples of wild relatives, synthetic forms, and introgression lines of common wheat (Triticum aestivum L.) were genotyped with diagnostic molecular markers that are linked to the leaf-rust resistance genes Lr9, Lr35, and Lr47. The Lr9 gene was identified in Aegilops umbellulata and Lr35 and Lr47 were identified in Ae. speltoides. The synthetic forms Avrolata and Avrodes, in which the genomes of Ae. umbellulata and Ae. speltoides, respectively, replaced the wheat D genome, contain the Lr9 and Lr35 genes. The genomes of the introgression lines that were developed using Ae. umbellulata lack Lr9. The Lr35 gene is present in one of the introgression lines that was derived from Avrodes. Some of the wild relatives and the introgression lines may contain additional leaf-rust resistance genes.