Performancel of the microplate bactraceTM EKISA technique for detection of foodborne Salmonella

The developmental BacTrace TM ELISA system which recognizes a common structural antigen (CSA-1) in the cell wall of target microorganisms was tested with pure cultures and naturally contaminated foods. The system readily detected all of the 104 Salmonella test strains but produced 38 (52.1%) false-positive reactions upon examination of 73 nonsalmonellae cultures. Citrobacter freundii, Escherichia coli, and Proteus mirabilis were primarily responsible for erroneous results. False-positive reactions were particularly prominent with high moisture foods