Characterization and molecular mapping of stripe rust resistance in wheat – Psathyrostachys huashanica introgression line H9015-17-1-9-6

AbstractStripe rust (yellow rust), caused by Puccinia striiformis Westend. f. sp. tritici (Pst), is one of the most destructive fungal disease in wheat(Triticum aestivum L.). Growing resistant cultivars is the best strategy to control stripe rust. The wheat introgression line H9015-17–1-9–6 was developed from an interspecific hybridization between the common wheat cultivar 7182 and Psathrostachys huashanica Keng (2n = 2x = 14, NsNs). H9015-17–1-9–6 has all-stage resistance to all known Chinese stripe rust races, including three widely virulent races: CYR32, CYR33 and CYR34. To identify stripe rust resistance genes in the introgression line, H9015-17–1-9–6 was crossed with the susceptible genotype ‘Mingxian169' and the F1, F2, F3 and BC1 generations were inoculated with Pst races under controlled greenhouse conditions. The results indicated that a single dominant gene in H9015-17–1-9–6, temporarily designated as YrH9015, confers resistance to race CYR32. Using bulked segregant analysis methods, we identifi...