The antimicrobial action of decamethoxinum substance at different pР values

This study considers the effect of pH on the specific activity of decamethoxinum substance under experimental conditions. It has been shown that the minimum inhibitory concentration (MIC) of this substance depends on the microorganism species and pH; the lowest MIC values for S. aureus, P. aeruginosa, E. coli are determined in the basic medium. The significant antimicrobial activity in both acidic and basic media was shown against S. paratyphi, and the antifungal effect against C. albicans increased in the basic medium. It has been determined that decamethoxinum inhibits formation of biofilms by E. coli and P. aeruginosa, the effect depends on the concentration pH 8.0±0.1 in the concentration of 10.0). Decamethoxinum-induced inhibition of the microorganism biomass accumulation is also pH-dependent. Thus, inhibition of the growth and reproduction of P. aeruginosa was observed in 6 hours of incubation with the substance at pH 6.3±0.4; inhibition of the vital activity of E. coli was registered in the basic medium (pH 8.0±0.1) in 1 hour of incubation; while inhibition of S. paratyphi was in 12 hours of incubation at pH 5.1±0.2; 6.3±0.4; 8.0±0.1. The most significant activity of decamethoxinum against the gram-positive bacteria (S. aureus) was revealed in 6 hours of incubation at pH 5.1±0.2. In the acidic medium mentioned the antifungal action against C. albicans was also observed with inhibition of the cell accumulation in 12 hours after the substance introduction.