Разработка методики контроля качества настойки чистотела большого гомеопатической матричной

Homeopathic matrix tinctures of greater celandine ( Chelidonium majus L .) obtained using freshly collected and desiccated raw material (roots) analyzed by GC - MS showed the presence of a combination of alkaloids including canadine, stylopine, homochelidonine, protopine, dihydronitidine, norchelerythrine, dihydroberbine, and norsanguinarine. Dihydroberbine, norchelerythrine, and norsanguinarine were detected only in tinctures obtained from desiccated raw material and not detected in the preparations from freshly collected row material. New methods for the quality control of homeopathic matrix tinctures of celandine root have been developed using qualitative chemical reactions and TLC on Sorbfil plates eluted in an ethyl acetate - ethanol - 0.1 M sodium hydroxide (6:3:2) solution with reference to the standard solutions of sanguirythrine (sanguinarine + chelerythrine) and berberine in ethanol. The spots (adsorption bands) on the TLC plates are detected with Dragendorff's reagent. Alkaloids are extracted from tinctures with chloroform. Quantitative estimation of the total alkaloid content (recalculated for sanguirythrine) was carried out by UV spectrophotometry at 278 nm. The total alkaloid content in homeopathic matrix tinctures obtained from freshly collected raw material varies within 0.015 - 0.032 %, and in those from desiccated raw material, within 0.028 - 0,081 %.