Effects of alcohol on expressions of 6 kinds of neurotransmitters mRNA in bone marrow mesenchymal stem cells of rabbits

Aim: To investigate the effect of alcohol on the mRNA expressions of fibrocyte growth factor(FGF),calcitontin gene-related peptide receptor(CGRPR),vasoactive intestinal peptide receptor(VIPR),nerve growth factor(NGF),substance P receptor(SPR) and peroxisom proliferator-activated receptor(PPAR)in bone marrow mesenchymal stem cells(BMSCs) of rabbits.Methods:BMSCs of rabbits were seperated and divided into two groups randomly.The cells of the experimental group were treated with 0.09 mol/L ethanol,and the cells of the control group were treated without ethanol.The mRNA expressions of neurotransmitters mentioned above in the cells were investigated by RT-PCR at Day 4 and 7.Results:The mRNA expressions of FGF,CGRPR,VIPR,NGF and SPR of the experimental group were significantly lower than those of the control group(Day 4:t=5.327,34.773,12.036,28.660 and 22.584,P0.01;Day 7:t=4.664,44.965,8.461,12.809 and 35.163,P0.01),but that of PPARγ mRNA increased (t=31.107 and 36.775,P0.001).Conclusion:Alcohol could inhibit the expressions of neurotransmitters mentioned above in BMSCs,which is involved in pathogenesis of the alcohol-induced osteonecrosis of femoral head.