Hybridation in situ sur coupes en paraffine : Nouvelle technique à l'institut national d'oncologie

In situ hybridization is a molecular technique which allows for in situ detection, on paraffin sections of tumoral specimens, of viral nucleic acid using complementary probes hybridizing with the viral genetic material present in the infected cells. This technique, which has been introduced for the first time in our country, is highly interesting as pathogenic, diagnostic and epidemiological tools detecting, without the need for RNA extraction, the causative agent in some diseases. This is indeed the case in some viral-induced cancers as the rhinopharynx one which is closely linked to the presence of Epstein-barr virus.