Determination of Four Residues of NAIDs in the Crucian

[Objective] To develop a novel method for the determination of four kinds of NSAIDs(non-steroidal anti-inflammatory drugs) residues including flunixin meglumine,meloxicam,ketoprofen and diclofenac sodium in the crucian by HPLC,assisting by microwave and ultrasound.[Method] The NSAIDs were extracted with the acidificated ethanol and purified by diatomite column,the compounds were eluted by methanol.[Result] The subsequent analysis of NSAIDs was achieved using acetonitrile-0.1% triethlamine(adjusted pH value to 3.5 with phosphate)(60∶40,by volume) as mobile phase with a flow rate of 0.8 ml/min at 30 ℃.The detection wavelength was set at 255 nm.The detection limit of four kinds of NSAIDs were 5-10 μg/kg(S/N=3) and the limits of quantitation(LOQ) were 15-30 μg/kg(S/N=10).The adding recovery rates were at the range of 72.5%-93.8% with RSD below 15%.[Conclusion] The method was simple,rapid,high sensitivity and specificity,and it could meet the qualitative and quantitative analysis requirements.