Abstract 15148: A Small Molecule Derived From Carboxyethylpyrrole Protein Adducts Promotes Angiogenesis in a Mouse Model of Peripheral Arterial Disease

Introduction: ω-(2-carboxyethyl)pyrrole (CEP) protein adducts are the end products of lipid oxidation associated with inflammation, and they are key players in orchestrating angiogenesis in pathological conditions such as tissue ischemia. We synthesized small molecules derived from CEP protein adducts and evaluated the angiogenic effect of the CEP analog, CEP03, in the setting of peripheral arterial disease (PAD). Hypothesis: We hypothesize that CEP03 will induce angiogenesis in a murine model of PAD. Methods: The angiogenic effect of CEP03 was assessed by in vitro analysis of primary human microvascular endothelial cell (EC) proliferation and tube-like formation in matrigel. The therapeutic potential of CEP03 was further evaluated in vivo in a mouse model of peripheral arterial disease by quantification of blood perfusion recovery and histological assessment of capillary density. Results: In the presence of CEP03, proliferation in vitro increased by 127±18% under hypoxic (1% O2) conditions, reaching simi...