FISH-AFLP analysis of genetic diversity of early-fruiting walnut cultivars.

16 early-fruiting walnut(Juglans regia L.) cultivars were developed in China in 1994.These new walnut cultivars preliminarily formed an improved variety system for Chinese walnut production.The FISH-AFLP analysis was conducted in order to investigate the sibship among the 16 Chinese early-fruiting walnut cultivars.9 primer combinations were used to amplify the genomic DNAs.Total 1 072 AFLP fragments were obtained,in which 946 are polymorphic markers and the average percent of polymorphic bands is 88.12%.Different genotypes were detected and unique bands were found.The genetic diversity and their sibship among Chinese early-fruiting walnut cultivars were investigated and their fingerprintings were established in the research.