Effects of Fuzi Lizhong Decoction on Content of ANP,Expression of pGC mRNA and Gastrointestinal Dynamics in Rats with Spleen Yang Deficiency Syndrome

Objective: To observe effects of Fuzi Lizhong decoction on content of ANP,expression of pGC mRNA and gastrointestinal dynamics in rats with spleen Yang deficiency syndrome. Method: Hundred clean grade Wistar rats were randomly divided into control group,model group,Fuzi Lizhong decoction low-dose group, middle-dose group and high-dose group 5 groups with 20 rats in each group. The control group was given ordinary diet while model group was administrated by incising brown adipose tissue(BAT),high fatty diet and cold enviroment stimulating. From the first day after incised BAT,model group was given high fatty diet for 3 weeks, and low-dose group,middle-dose group and high-dose group were ig respectively with Fuzi Lizhong decoction(10, 20,40 g·kg- 1) based on model group,once a day for 3 weeks. On the first day after 3 weeks the activities of jejunum muscle was observed andcolon was collected to examine content of ANP by ELISA and expression of pGC mRNA by RT-PCR. Result: Compared with the control group,in the model group,content of ANP and expression of pGC mRNA reduced(P 0. 05 or P 0. 01),frequency of slow wave reduced significantly(P 0. 01) while occurrence frequency of fast wave,maximum amplitude,spike burst per cluster increased(P 0. 05 or P 0. 01),both amplitude of longitudinal muscle contraction and amplitude of circular muscle contraction enhanced significantly(P 0. 01). Compared with the model group,in high-dose group,content of ANP and expression of pGC mRNA increased(P 0. 05 or P 0. 01). in middle-dose group frequency of slow wave increased(P 0. 05) while occurrence frequency of fast wave,maximum amplitude,spike burst per cluster reduced(P 0. 05 or P 0. 01),in high-dose group frequency of slow wave increased significantly(P 0. 01) while occurrence frequency of fast wave,maximum amplitude,spike burst per cluster reduced significantly(P 0. 01),in middledose group amplitude of longitudinal muscle contraction reduced(P 0. 05) while in high-dose group both amplitude of longitudinal muscle contraction and amplitude of circular muscle contraction reduced significantly(P 0. 01). Conclusion: Fuzi Lizhong decoction could increase content of ANP and expression of pGC mRNA to restore gastrointestinal dynamics,meantime,regulate the reabsorption of water and NaCl,which perhaps is one of its mechanisms of improving symptoms of diarrhea.