Analysis of pinot cultivars by microsatellite markers

Molecular markers are widely used because of their wide range of applications. The identification of the cultivars and clones of Pinot by microsatellite methods according to the literature, present difficulties (Regner et al., 2006; Stenkamp et al., 2009). Hocquigny et al. (2004) carried out SSR analyses among Pinots. Polymorphism analysis revealed that 65% shared the most frequent genotype. Moreover, the variant clones showed at least 96% similarity with this genotype. Based on our earlier experience, 7 ?Pinot gris?, 4 ?Pinot noir? clones and ?Pinot blanc? were analysed for 16 (VVS2, VMC5E9, VMC3D12, VVIM10, VMC5G8, VMCNG1E1, VMC1F10, VMC2H4, VMC8A7, VMC7G3, VVMD28, VrZag21, VrZag79, VMC1C10, VrZag25, Scu06vv) microsatellite loci. A dendrogram was constructed using the shared allele method for estimates of genetic similarity between pairs, and the neighbour joining method for clustering. Based on our results, it can be established that the Pinot clones all showed high similarity. The ?Pinot gris? clones bred in Badacsony, Hungary (B. 10, B. 10/5, B. 10/10) formed a group and showed the highest similarity with ?Pinot gris? 34 from Romania. The other ?Pinot gris? clones formed another group with Pinot noir C-162. These clones all originated from western Europe (Germany, France). These genetic differences could be traced back to the different geographic origin of the different clones.