A Highly Sensitive and Group-targeting Aptasensor for Total Phthalate Determination in the Environment

Abstract As the most widely used and typical kind of plasticizers, phthalate esters (PAEs) have become one of the most common environmental pollutants in the world. Therefore, it is necessary to develop a rapid and convenient method for determining the total amount of PAEs. Herein, a molecularly tailored broad-spectrum aptamer that can recognize multiple similarly structured total amounts of PAEs (TP) and bind them with high affinity has been successfully fabricated. Mfold (multiple folding) secondary structure simulation and molecular truncation were both utilized to obtain the most effective binding region from the parental full-length (39-mer) aptamer. The results show that the PAE-binding affinity of the truncated 24-mer aptamer produced by removing nonessential flanking nucleotides was improved by 1.5-fold. The linear range of TP detection is 0.003-10 μg/L, and the limit of detection is 1 ng/L. Notably, our study provides new insights into the group-targeting identification of certain pollutants and determination of their total amounts, exhibiting great potential for practical applications.