1-磷酸－神经鞘氨醇预防化疗大鼠卵巢功能损害的实验研究

[Objective] To study the effect of sphingosine-1-phosphate (S1P) against cyclophosphamide (CTX) induced gonadotoxicity in female rats. [Methods] Forty SD female rats were divided randomly into four groups control (received normal saline (NS), CTX, S1P+NS, S1P+CTX respectively. All rats were killed between the first and the second week in the diestrus stage after stopping medicines to compare the number of the primordial follicles, the primary follicles and the growth follicles. The expression of the Bcl-2 and Bax mRNA in the ovaries were examined by the RT-PCR. [Results] The number of primordial follicles, primary follicles and growth follicles in the S1P+CTX group was close to that in the control group and the S1P+NS group (P＞0.05). But the number of all kinds of follicles was decreasing in the CTX group, and there were significant differences between that of these groups (P＜0.01).The levels of the Bcl-2 mRNA in the two S1P groups were significantly higher than that in the CTX group and the control group, but levels of the Bax mRNA were on the contrary (P＜0.01). The level of the Bcl-2 mRNA in the CTX group was significantly lower than that in the S1P groups and the control group, but the level of the Bax mRNA was on the contrary (P＜0.01). [Conclusion] S1P could reduce the cyclophosphamide-induced ovarian damage in rats, and the role of its against apoptosis might through regulating the expression of the Bcl-2 and Bar mRNA in the ovary.