299 Differential abundance of mirnas circulating in peripheral blood of patients with class iv lupus nephritis

Background and aims Among the organs involved in-the SLE, we can highlight the renal damage, as the largest contributor to mortality in SLE patients is estimated that nearly-50% of SLE develop kidney disease in the first years of diagnosis. Class-IV lupus-nephritis (LN-IV) is the class of lupus nephritis most common in Colombian-patients with systemic-lupus-erythematosus. MicroRNAs are important molecules involved in the pathogenesis of LN. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral-blood of Colombian-patients with LN class IV. Methods an observational case-control, cross-sectional. Patients-diagnosis by biopsy class IV lupus-nephritis was compared with patients without nephritis, and healthy-individuals was raised. These were extracted venous blood, which total RNA, which was subsequently sequenced. it was Compared Against the miRBase and Ensembl database. Differential gene expression analysis was Carried Out with edgeR and Functional analysis functional analysis was done with DIANA-miRPath.Was used as variables of selection Fold-Change(≥2 o ≤−2) and False-Discovery-Rate (0.05). Results We identified 24 circulating microRNAs with diference abundace that LNN or CTL, two of these microRNAs miR-107-3p and miR375-3p are described for first time to lupus nephritis. Conclusions This changes in the abundace of miRNA, it implies alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease-biomarkers for early-diagnosis of LN.