Multilocus sequence typing of Campylobacter jejuni and Campylobacter coli isolates from poultry, cattle and humans in Nigeria.

Aims To determine the genetic diversity of Campylobacter jejuni and Campylobacter coli isolates from Nigeria and to identify the association between multilocus sequence types and hosts (poultry, cattle and humans). Methods and Results Isolates were identified using multiplex PCR assays. Multilocus sequence typing (MLST) was used to determine the genetic diversity of 36 Campylobacter jejuni and 24 Campylobacter coli strains isolated from poultry, cattle and humans. Of the 36 C. jejuni genotyped, 21 sequence types (ST) were found, 9 (43%) were new while out of the 24 C. Coli isolates genotyped, 22 STs were identified with 14 (64%) being new. The most prevalent sequence type was ST1932 followed by ST1036 and ST607 while the prevalent clonal complexes were CC–828, CC–460 and CC–353. Conclusions Campylobacter isolates from Nigeria were found to be diverse with novel genotypes. There was overlap of CC-828, CC-460 and CC-353 between the poultry, cattle and human isolates. Genetic exchange was also detected in 2 of the C. coli isolates. Significance and impact of the study This study highlights the genetic diversity of Campylobacter strains in Nigeria, demonstrating that C. jejuni and C. coli isolates are diverse and have both local and global strains. The predominant sequence types and clonal complexes found in this study differ from other countries; this exemplifies that different predominant Campylobacter populations exist between countries. This article is protected by copyright. All rights reserved.