Additional file 8: of Dietary arachidonic acid increases deleterious effects of amyloid-β oligomers on learning abilities and expression of AMPA receptors: putative role of the ACSL4-cPLA2 balance

Modification of hippocampal and cortical presynaptic protein SNAP25 expression induced by ARA diet. Immediately after the probe test, mice were killed, and synaptosomes were prepared from the cortex and hippocampus. Representative immunoblots of cortical (a) and hippocampal (b) SNAP25 from OLE or ARA mice after NaCl or Aβ42 injections are shown. Densitometric analyses were performed to determine signal intensities normalized to β-tubulin, and data are expressed as the percentage of control OLE mice injected with NaCl. Results are shown as mean ± SEM of immunoblots performed for all animals (OLE groups n = 2, n = 4; ARA groups n = 2, n = 6). (PPTX 59 kb)