Agrobacterium-mediated Transformation of Rice ‘Ilmibyeo’ using HPT Selection Marker Gene

This study was conducted to produce the transgenic plant of rice. We obtained Agrobacterium AGL1 harbaring pCambia1300 vector with HPT gene. We carried out PCR analysis of 22 ea putative transgenic rice to investigate transformed lines. The 3 ea transgenic lines were detected insertion of HPT gene. Transgenic lines selected from PCR analysis were performed by Southern blot. From Southern blot, we obtained that two transgenic lines detected single band. We are going to study the method improving of cotransformation as well as transformation efficiency in rice.