Supplemental Information Adiponectin Enhances Insulin Sensitivity by Increasing Hepatic IRS-2 Expression via a Macrophage-Derived IL-6-Dependent Pathway

Male C57BL/6J mice were anesthetized with pentobarbital sodium, and the liver was perfused in situ via the portal vein with the perfusion medium, followed by the collagenase medium. The liver was removed and the dissociated cells were filtered through Cell Strainer (BD Falcon) into Williams Medium E (GIBCO) containing 10% (vol/vol) fetal bovine serum, and plated onto collagen I-coated dishes (IWAKI Glass). For culture, the medium was supplemented with 100 nM dexamethasone and 1 nM insulin. The perfusion medium: 0.8% NaCl, 5mM KCl, 0.5 mM NaH