AN IMPROVED METHOD FOR THE ISOLATION OF SUPERCOILED PLASMID DNA

Rapid methods of small-scale preparation of plasmid DNA are very common in molecular biology and genetic engineering. Several procedures are in vogue at present to produce plasmid DNA suitable ments. Mini preparation of plasmid DNA by alkaline lysis' and boiling? methods contain chromosomal DNA and require cumbersome phenol, chloroform extractions. Most of the commercial kits that are available for mini-preparation of pure plasmid DNA are very expensive. For various molecular biology experience.