Structural analysis of a developmentally regulated sequence encoding for a cysteine proteinase in Dictyostelium discoideum

A developmentally regulated DNA sequence was isolated from a Dictyostelium discoideum genomic library by cross hybridization with a cDNA clone of cysteine proteinase I. The genomic fragment represents a different but related gene, designated cysteine proteinase II. This sequence is unique in the genome and it hybridizes to a polyA+ RNA of 1.5 kb length, which is produced after the aggregation period.