Modeling the formation of microorganism-derived dissolved organic nitrogen (mDON) in the activated sludge system

Abstract Microorganism-derived dissolved organic nitrogen (mDON) represents a significant and inevitable portion of dissolved organic nitrogen (DON) in the wastewater biotreatment processes. In the existing method, mDON concentrations are indirectly measured by the values of DON concentrations from the reactors with DON-free influent. However, this becomes problematic when influent contains DON. Especially when the real wastewater is involved, the paucity of the direct methods to quantitatively measure mDON is a major barrier to further research. This limitation is due to the difficulty of segregating mDON from the other nitrogenous organics, e.g., influent DON. In this study, we propose the ASM-mDON model based on ASM #1, which incorporates the production and consumption of mDON in the activated sludge processes to predict the mDON concentrations. In four independent lab-scale tests, our model was established and calibrated to obtain the accurate values of mDON (R2 = 0.929, p