Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae.

S.M. Da Silva,Lindsay Vang,Nathanael D. Olson,Steven P. Lund,A.S. Downey,Z. Kelman,Marc L. Salit,Nancy J. Lin,Jayne B. Morrow

Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae.

2016

S.M. Da Silva
Lindsay Vang
Nathanael D. Olson
Steven P. Lund
A.S. Downey
Z. Kelman
Marc L. Salit
Nancy J. Lin
Jayne B. Morrow

Aims We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR).

Keywords:

Computational biology
DNA extraction
Saccharomyces cerevisiae
Microbiology
Real-time polymerase chain reaction
Biology

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