EP582 Association between changes in relative expression of genetic loci encoding transcription factors and age and endometrial cancer grade

Introduction/Background Transcription factors encoded by the OCT4 and SOX2 genes are involved in the regulation of human embryonic stem cell function and may play an important role in tumor progression. Mutant versions of the Myc gene are found in many tumors. Our purpose was to study relative expression of genetic loci encoding transcription factors in intact and pathological tissue of the uterine body. Methodology Biopsy tissues of the uterus (tumor - TT and intact - IT) were studied in 27 patients aged 38–72 years with histologically verified endometrial cancer (EC). Relative expression of 3 genetic loci encoding OCT4, SOX2 and c-Myc transcription factors was determined by RT-qPCR. The primer design for RT-qPCR was performed using NCBI GenBank DNA reference sequences. ACTB was used as the reference gene. Results Patients with G1 EC, 45-68 years, showed an increased SOX2 expression (by 178%, p Conclusion The maximal increase was observed in the OCT4 gene expression, confirming its status as a marker of undifferentiated cells. In tumors of higher grades, changes in OCT4, SOX2, and c-Myc expression affected uterine IT, however with less intensity, especially in later stages of the disease. Disclosure Nothing to disclose.