Partial sequence analysis of two isolates of Cucurbit aphid-borne yellows virus from Hubei and Yunnan in China.

Two isolates of Cucurbit aphid-borne yellows virus(CABYV)were obtained from naturally infected cushaws with yellowing symptom from fields in Kunming city of Yunnan and Wuhan city of Hubei.A pair of primers were designed based on a published CABYV sequence,and the expected fragments of about 1375 nt were amplified by RT-PCR and cloned into pMD19-T in DH5α,respectively.The recombinant clones were identified by PCR reaction and subsequently sequenced(GenBank accession number are EF488996 and EF488997).The obtained sequences started within a single open reading frame which was expected to encode a part of the RdRp of 191 amino acids by 576 nucleotides,an intergenic non-coding region(NCR)of 199 nucleotides and followed by complete capsid protein(CP)of 199 amino acids encoded by 600 nucleotides.The identities of nucleotide and deduced amino acid sequences of CP gene among CABYV-HB,CABYV-YN and other nine previously reported isolates from France,Italy,Spain,Beijing,Shanghai ranged from 93.1% to 98.5% and 91.4% to 98.5%,respectively.