Identification of parentage of ornamental crabapple seedlings using AFLP markers.

The polymorphism was analyzed by AFLP in 38 Ornamental Crabapple seedlings from female parent,Malus 'Royalty',and 5 possible male parents.Results showed that the total 349 informative AFLP fragments were generated using the selected 9 EcoRⅠ-MseⅠ primer combinations with high resolution capacity.Among them there were 303 polymorphic fragments with a polymorphism percentage of 86.8%.According to the characteristic bands of electrophoretogram and Cluster Analysis,the male parents of 19,7 and 3 seedlings were identified to be Malus 'Jewelberry',Malus spectabilis Borkh.and Malus 'Strawberry Parfait' respectively.The remnant 9 seedlings could not be confirmed or identified.