Vergleichende Analyse der nach den Extraktionsmethoden von Kirby und Georgiev aus Mäuseleber extrahierten RNS-Fraktionen / Analysis of the RNA Fractions of Mouse Liver: Comparison of the Extraction Methods of Kirby and Georgiev

Ribonucleic acids labeled with ¹⁴C- or ³H-orotic acids, respectively were extracted from aliquots of mouse liver homogenates according to the cold-phenol-method of Kirby or the method of Georgiev employing different temperature steps.This comparative analysis showed that only ca. 50% of the total labeled RNA was extracted by the Kirby method. Especially the high molecular D-RNA and R-RNA were not sufficiently extracted, as was demonstrated by hydroxyapatite- and methylalbumin-Kieselgur column chromatography.Reextraction of the tissue at 65 °C first extracted by the Kirby method resulted in a recovery of most of the missing nucleic acid species.Therefore, results obtained by the Kirby and Georgiev methods will be different in regard to the nucleic acid composition. These findings could explain contradictary results about this problem in literature.