The effect of astragalus calycosin monomer to TNF-α induced human umbilical vein endothelial cells ET-1/NO

Objectives To investigate the astragalus calycosin monomer TNF - α induced human umbilical vein endothelial cells ( ECV - 304) of nitric oxide ( NO),endothelin - 1 ( ET - 1 ) effects.Methods DMEM culture medium at 37 ℃,5％ CO2 conditions on the ECV304 cell line was amplified to a sufficient number,the adjusted cell density of 1 × 105,and divided into 7 groups:A group:control group,without any inducer; B group:TNF-α (40ng/ml) induced group; C group:TNF-α (40ng/ml) + losastan (10 ～5mol/l) ; D group:TNF-α(40ng / ml) + calycosin (0.1mg/ml) ; E group:TNF - α (40ng/ml) + calycosin (lmg/ml) ; F group:TNF -α (40ng/ml) + calycosin (10mg/ml); G group:TNF-α (40ng/ml) + calycosin (20mg/ml).Were incubated for 6 hours,supernatant was collected after 24 hours,the supernatant by ELISA detection of nitric oxide (NO),endothelin 1 ( ET - 1 ) levels.Results ① compared with the control group,TNF - α reduced the ECV - 304 NO levels ( P ＜0.001 ),increased ET - 1 levels( P ＜ 0.001 ) ; ② losartan increased ECV - 304 NO levels( P ＜ 0.001),reduced the ET-1 levels( P ＜0.001) ; ③ calycosin (0.1 mg/ml～ 10mg/ml) ECV -304 cells can promote NO production( P ＜0.001 ),reduced ET- 1 production( P ＜ 0.001 ) ; ④ losartan potassium and calycosin (20mg/ml) ECV -304 cells for ET - 1 and NO levels not significantly different( P ＞ 0.05 ) ; ⑤ calycosin concentration (at 0.1 mg/ml -20mg/ml inside) and ECV-304 ET- 1 secretion was negatively correlated (r =- 0.02,P ＜ 0.001 ) ; and ECV -304 NO secretion was positively correlated (r =0.0075,P ＜0.001 ).Conc -lusions Astragalus calycosin monomer can promote TNF - α induced endothelial cell NO secretion,inhibition of ET - 1 secretion,and within a certain concentration (0,1 mg / ml ～ 20mg/ml) in a concentration dependent manner. Key words: Tumor Necrosis Factor ;  Umbilical Veins ;  Isoflavones ;