Nucleic acid detection method and system
2003
For in using nucleic acid amplification methods for the detection of nucleic acids, nucleic acid amplification method using a direct, so it takes shorter time measurement, such as a gene integrated and effective inspection system, the processing of biological samples taken in through the collect biological sample introduced into the homogenization step or means for suppressing the activity of a nucleic acid decomposition step, is not isolated and purified nucleic acid components from a biological sample. Introduction means decomposition activity to inhibit the nucleic acid is at an acidic pH, particularly is carried out at pH2.5 ~ pH5, the reaction was amplified by using the nucleic acid obstruction and / or nucleic acid interactions constituents salt treatment, separation and purification of nucleic acid components of the nucleic acid amplification is not performed directly.
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