Exposure of Bisphenol‐A in breast cancer patients‐quantitatively assessed by a sensitivity‐enhanced high performance liquid chromatography coupled with fluorescence detector: A Case‐Control Study

A simple, reproducible and sensitive liquid chromatography (HPLC) method has been developed and validated for estimation of Bisphenol A (BPA) in human urine. A simple liquid-liquid extraction technique was used in BPA sample preparation. The analyte was chromatographed on a Shimadzu prominence HPLC system using isocratic mobile phase conditions at a flow rate of 0.500 mL/min and hypersil gold C18 column maintained at 40°C. Quantification was performed on a fluorescence detector set at excitation 275 nm, emission 313 nm and bisphenol-B was used as internal standard. The total run time was 8 min. The method was found to have acceptable sensitivity, selectivity, accuracy (98.82 % - 103.64 %), precision (1.17-5.36) and stability in the validation experiment carried out as per the USFDA guideline. The method was sensitive as low as the concentration level of 0.50 ng/mL. The applicability of the validated analytical method has been established in human patient urine samples. The mean human urine BPA concentration were in control group 1.18±2.11 ng/mL and 5.76±6.00 ng/mL in the patient group (P<0.001). Therefore, this method could be considered as an alternative for routine bio-monitoring of BPA which is less expensive and feasible in resource poor settings.