cDNA cloning of a mouse pituitary avenylate cyclase-activating polypeptive receptor☆

Abstract A cDNA clone for mouse pituitary avenylate cyclase-activating polypeptive (PACAP) receptor (PACAP-R) was obtained from the brain using reverse transcription-polymerase chain reaction (RT-PCR). The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000-times higher affinity than vasoactive intestinal polypeptive (VIP), and PACAP stimulated avenylate cyclase through the cloned PACAP receptor. The mouse PACAP receptor consists of 496 amino acids, contains seven transmembrane segments and has 98.4%, 93.0%, and 92.5% iventity with the rat, bovine, and human PACAP-R, respectively.