Bacterial invasion of pulp blood vessels in teeth with symptomatic irreversible pulpitis.

INTRODUCTION This study described the degenerative changes and infection patterns of the pulp tissue associated with symptomatic irreversible pulpitis. METHODS The material consisted of 32 extracted teeth with untreated deep caries that were clinically and histologically diagnosed as with irreversible pulpitis and were part of the histopathologic collection of one of the authors. Controls consisted of intact teeth with normal uninflamed pulps and teeth with reversible pulpitis. Teeth were processed for histopathologic and histobacteriologic analyses. RESULTS All teeth with irreversible pulpitis showed areas of severe acute inflammation, necrosis, microabscesses and bacterial infection in the pulp chamber. These areas were surrounded by a chronic inflammatory infiltrate and, at the distance, the pulp tissue was often uninflamed. Bacteria were also seen in the areas surrounding the necrotic foci, both as scattered cells through the extravascular space and at varying numbers within the blood vessels lumen. The number of bacteria and the density of the intravascular bacterial aggregations varied considerably. In one third of the cases, bacteria occurred in the lumen of venules in areas at a considerable distance from the necrotic focus, in the coronal third of the root. No intravascular bacteria were seen in the middle and apical segments of the canal. No bacteria were found in the pulps of any of the control specimens. CONCLUSION Bacterial invasion and colonization of necrotic areas were observed in the pulps of all teeth with caries exposure and symptomatic irreversible pulpitis. Bacterial penetration of blood vessels occurred in all cases, suggesting that this may be an important mechanism of spread of bacterial infection through the pulp tissue in an endodontic infection.