Study on the diversity of non-Saccharomyces yeasts in Chinese wine regions and their potential in improving wine aroma by β-glucosidase activity analyses

This work investigated the diversity of non-Saccharomyces yeasts in main Chinese wine producing area, and discuss their potential in wine fermentation through analyzing their β-glucosidase activity. Grapes from 44 vineyards of 9 regions were detected and a total of 395 non-Saccharomyces were identified and categorized into 16 genera, 28 species. In which, 85 non-Saccharomyces yeasts were primarily screened based on pNPG method and Bradford method, and then evaluated by β-glucosidase environmental adaptability, substrate affinity and enzyme activity. Two selected strains were then inoculated individually or sequentially with commercial Saccharomyces cerevisiae into Gewurztraminer grape juice to detect the physiochemical indexes by HPLC and aroma compound by HS-SPME/GC-MS-FID. The results showed both non-Saccharomyces sequential inoculations increased the total aroma content, while the Candida glabrata strain D18 significantly increased the typicality and complexity of the floral and fruity aroma in Gewurztraminer wines, demonstrated its potential in wine fermentation.