Characterization of 13 microsatellite loci for the Pygmy Marbled Newt Triturus pygmaeus (Salamandridae)

We have developed thirteen polymorphic microsatellite loci for the newt Triturus pygmaeus using an enriched-library approach. We have detected 171 alleles in 60 individuals genotyped (mean number of alleles per locus was 13.15) in two different populations in the Donana National Park, Southwestern Spain. Expected heterozygosities ranged from 0.16 to 0.956 in one population (Mata de los Dominguez) and from 0.1 to 0.959 in the other population (Navazo de la Higuera). The levels of polymorphism of the developed markers render them readily applicable for population genetic studies of diversity, structure, and migration.