EFFECT OF ABA ON SEED DEVELOPMENT OF CASTANOPSIS CARLESII AND C. KAWAKAMII

The method of ELISA was used to determine ABA contents during different seed developmental stages of C.carlesii and C.kawakamii , sensitivity of seed to different concentrations of the exogenous ABA and accumulative reaction to storage protein by means of radial isotopic tracing. The results showed that during the development of C.carlesii and C.kawakamii seeds, endogenous ABA contents increased obviously, both peaked at 1648 ng/g ( C.carlesii ) and 1468 ng/g ( C.kawakamii ) on 5, October, and then the contents gradually decreased. When the seeds fully matured, only one ninth of the c (ABA) could be determined. As the sensitivity to exogenous ABA decreased during development, 10 -4 mol L -1 ABA could inhibit the germination of the seeds in which c (ABA) could not reach peak. However, 10 -2 mol L -1 ABA could not inhibit the germination of the fully matured seeds. c (ABA) could not affect synthesis and accumulation of storage protein at the early developmental stage, but could accelerate them at the middle and late stages. ABA in regulation of the synthesis and accumulation of storage proteins was reflected by the transcriptional level.