[Effect of c-myb on hCG-induced testosterone secretion in isolated rat Leydig cells].
2004
The present study was carried out to investigate the effect of antisense c-myb oligodeoxynucleotides (ODN) on hCG-induced
testosterone secretion in isolated rat Leydig cells. The effects of cAMP, Ca~(2+) and cycloheximide (CYX) on c-Myb protein expression and
testosterone secretion were also observed. The results showed that antisense c-myb ODN inhibited hCG-induced testosterone secretion of
isolated rat Leydig cells in a dose-dependent manner.At the same time, integral optical density immunostaining of Myb in Leydig cells was
also remarkably reduced. Nonsense tat ODN had no effect on Leydig cells. Further experiments showed that dbcAMP (100μmol/L) obviously
increased hCG-induced testosterone secretion and integral optical density (IOD) immunostaining of Myb in Leydig cells. Verapamil (10μmol/
L), a Ca~(2+) channel blocker, and cycloheximide (50μg/ml), a protein synthesis inhibitor, reduced the immunostaining of c-Myb, and also lowered
hCG-induced testosterone secretion in isolated rat Leydig cells. The results indicate that c-myb closely correlates with hCG-induced testoster-
one secretion, and that cAMP and Ca~(2+) -dependent pathway participates in the expression of protooncogene.
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