Efecto de Estimulaciones Infrarrojas sobre Hepatoesteatosis Microvesicular Alcohólica de Ratas

24 ratas hembras de 4 meses de vida con peso aproximado de 250 gramos fueron divididas en dos grupos de animales, A y B. Ambos grupos se mantuvieron con pellet y solucion de alcohol 40% durante 60 dias generandoseles una hepatoesteatosis microvesicular. Los higados de los animales pertenecientes al grupo B fueron estimulados con laser infrarrojo 6 J/cm2 durante 15 dias consecutivos. Posteriormente, las ratas fueron sacrificadas y se extrajeron muestras de higado y luego procesadas para microscopia electronica de transmision. De ambos tipos celulares se obtuvieron microfotografias electronicas de transmision con aumentos finales de 8.500 X, las cuales fueron sometidas a estudios morfometricos para determinar fracciones volumetricas de los siguientes componentes celulares: Reticulo endoplasmatico rugoso (RER), mitocondrias, inclusiones lipidicas y de glicogeno, eu y heterocromatina. De igual manera se cuantificaron las areas celulares y nucleares. Del analisis de los resultados entre hepatocitos esteatosicos e irradiados se visualiza que existen diferencias en todos los componentes celulares cuantificados y se concluye que los efectos de la estimulacion infrarroja con dosis de 6 J/cm2 provoca en los hepatocitos con esteatosis microvesicular transformacion en su ultraestructura y en su morfologia, fundamentalmente en la disminucion acentuada de las infiltraciones lipidicas hasta en un 80% situacion que se traduciria, en una variacion funcional, representando de esta manera un efecto evidente que estas inducciones infrarrojas generan. 24 four-month-old female rats weighing approximately 250 grams were divided into two groups labeled A and B. Both groups were fed pellets and a 40% alcohol solution for 60 days, which caused a microvesicular hepatic steatosis. The livers of the animals in Group B were stimulated with 6 J/cm2 of infrared laser for 15 consecutive days. The rats were then sacrificed and samples of both steatosic liver and liver stimulated with infrared inductions were extracted for immediate processing via transmission electron microscopy.From both cell types transmission electron microphotographs were obtained at magnifications of 9500 X; these were subjected to morphometric studies to determine volumetric fractions of the following cell components: rough endoplasmic reticulum (RER), mitochondria, lipid and glycogen inclusions, euchromatin and heterochromatin. Likewise, cell and nuclear areas were quantified. Analysis of the results between steatosic and radiated hepatocytes revealed notable differences in all the cell components quantified. It is concluded that the effects of infrared stimulation with a dose of 6 J/cm2 brings about in the steatosic hepatocytes a microvesicular transformation in their ultrastructure and morphology, fundamentally in the considerable decrease in lipid infiltrations to 80%, which ultimately translates into a functional variation, thus representing an obvious impact produced by these infrared inductions.