选择性环氧合酶－2抑制剂对胃癌细胞Bgc-823增殖的影响

Objective To investigate the effect of selective cyclooxygenase-2 inhibitors (SCIs) meloxicam and celecoxib on the proliferation and apoptosis of human gastric carcinoma cell line Bgc-823. Methods The number of Bgc-823 cells was determined by MTT assay. Tumor apoptosis and cell cycle were studied by flow cytometry. Results Meloxicam and celecoxib inhibited the proliferation of Bgc-823 cells in both dose-dependent manner and time-dependent manner. Celecoxib has more potent effect than meloxicam. Celecoxib could induce apoptosis of Bgc-823 cells, which were blocked in G0/G1 phase. The apoptosis rate increased with the rising concentration of celecoxib, and the proportion of Bgc-823 cells in G0/G1 phase increased. Conclusions SCIs can inhibit cell proliferation in Bgc-823 cells in both concentration-dependent manner and time-dependent manner. The mechanism may be dependent on the inhibition of COX-2 expression. SCIs affect cell cycles and induce Bgc-823 cell apoptosis in dose-dependent manner.