Fluorescence imaging analysis of taxol-induced ASTC-a-1 cell death with cell swelling and cytoplasmic vacuolization
2008
Taxol (Paclitaxel), an isolated component from the bark of the Pacific yew Taxus brevifolia, exhibits a broad spectrum of
clinical activity against human cancers. Taxol can promote microtubule (MT) assembly, inhibit depolymerization, and
change MT dynamics, resulting in disruption of the normal reorganization of the microtubule network required for
mitosis and cell proliferation. However, the molecular mechanism of taxol-induced cell death is still unclear. In this
report, CCK-8 was used to assay the inhibition of taxol on the human lung adenocarcinoma (ASTC-a-1) cells viability, confocal fluorescence microscope was used to monitor the morphology changes of cells with taxol treatment. We for the
first time describe the characteristics of taxol-induced cells swelling, cytoplasmic vacuolization and cell death. Taxol
induced swelling, cytoplasmatic vacuolization and cell death without cell shrinkage and membrane rupture. These
features differ from those of apoptosis and resemble the paraptosis, a novel nonapoptotic PCD.
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