Observation of the Feline Intestinal Epithelial Cell Infected with Toxoplasma gondii

Small intestine samples of neonatal cat were aseptically collected from the jejunum-ileum region and digested with collagenase Ⅺ/dispase Ⅰ. Immunohistochemistry results showed that feline intestinal epithelial cells were successfully isolated and could be cultured. Cytokeratin was positive in the cytoplasm of feline intestinal epithelial cells.The cells were infected with the bradyzoites of Toxoplasma gondii Prugniaud strain, and the rupture of the cells was observed on the 72 nd day post-infection. The sexual stage of T. gondii did not occur, however.