Optimization of the enzymatic production of 20(S)-ginsenoside Rg3 from white ginseng extract using response surface methodology
2009
The optimization of the conversion of ginseng saponin glycosides to 20( S )-ginsenoside Rg 3 by enzymatic transformation was carried out using response surface methodology (RSM) based on a 2 3 factorial central composite design. The production of 20( S )-ginsenoside Rg 3 using several commercial enzymes indicated that the enzyme Cellulase-12T was the most efficient at producing 20( S )-ginsenoside Rg 3 . To optimize the enzymatic production of 20( S )-ginsenoside Rg 3 , response surface methodology was applied to determine the ideal amount of white ginseng extract, Cellulase-12T and reaction time. These results indicate that white ginseng extract (1.67%) treated with Celluase-12T (3.67%) for 72 hours had 4 times the quantity of 20( S )-ginsenoside Rg 3 compared to commercial white ginseng extract.
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