Fluorescence immunoassay detection method and kit for HER-2

The invention relates to fluorescence immunoassay detection method and kit for HER-2. The method comprises the following steps: preparation of a probe fixing pad; preparation of an immunoassay test strip; preparation of sample diluent; and inspection of the sample. HER-2 monoclonal antibody fluorescence latex particles and goat anti-rabbit IgG fluorescence latex particles are mixed and then diluted by using gold diluent, and the diluted mixture is sprayed on glass fiber so as to prepare the probe fixing pad; and a detection line is coated by an HER-2 monoclonal antibody, and a control line is coated by goat anti-rabbit IgG so as to prepare the fluorescence immunoassay test strip. The detection kit provided by the invention comprises a PVC liner plate, a sample pad, the probe fixing pad, a cellulose nitrate membrane and absorbent paper. The probe fixing pad is prepared by mixing and drying the HER-2 monoclonal antibody fluorescence latex particles and the goat anti-rabbit IgG fluorescence latex particles. Thus, the fluorescence immunoassay detection method and kit have the advantages of high sensitivity, high accuracy, simple operation and low cost.