Effects of benzene, quercetin, and their combination on porcine ovarian cell proliferation, apoptosis, and hormone release
2019
Abstract. We hypothesized that the environmental contaminant benzene and the plant
antioxidant quercetin may affect ovarian cell functions and that quercetin
could offer protection against the adverse effects of benzene. This study
aimed to examine the action of benzene, quercetin, and their combination on
porcine ovarian granulosa cell functions. We elucidated the effects of
benzene (20 µ g mL −1 ), quercetin
(at the doses 0, 1, 10, 100 µ g mL −1 ), and their combination
on ovarian granulosa cell functions (proliferation, apoptosis, and hormone
release) in vitro using immunocytochemistry and enzyme immunoassay
respectively. Benzene alone stimulated proliferation, apoptosis, and oxytocin
release and inhibited progesterone and prostaglandin F release. Quercetin
alone inhibited proliferation, apoptosis, and stimulated oxytocin release
but did not affect progesterone and prostaglandin F release. When used in
combination with benzene, quercetin promoted the inhibitory effect of benzene
on progesterone release. Overall, these data suggest that benzene and quercetin have direct stimulatory and
inhibitory effects, respectively, on basic ovarian
functions. Moreover, no protective action of quercetin against the effects of
benzene was found. Rather, it was found to enhance the effect of benzene on
progesterone release. Therefore, quercetin cannot be considered for
preventing or mitigating the effects of benzene on reproductive processes.
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