Aβ(下标 1-42)对神经干细胞的增殖和分化的影响

Primary neural stem cells culture were prepared from 1 day neonatal ICR mouse. The brain tissue was mechanically separated, and neural stem cells were isolated and obtained. The purity of primary cell is more than 95% after identifying by immunofluorescence and RT-PCR. It is find that the fourth passage cells were similar to primary neural stem cells by growth curve, which showed the fourth passage neural stem cells still owned strong growth capability. For testing the effects of Aβ on the proliferation of neural stem cells, we exposed neural stem cells to 0.01μmol/L, 0.1μmol/L, 1μmol/L, 2.5μmol/L oligomeric Aβ1-42 for 8 h, 24 h and 48 h in vitro respectively. The results indicated the proliferation declined in a dose-and time-dependenc. For testing the effects of Aβ1-42 on the differentiation of neural stem cells, we made a statistic of trans-differentiation percent by double blind method, which showed Aβ1-42 had not obvious effection to neural stem cells' differentiation in a dose-and time-dependence.